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Metamorphosis is the fourth studio album by Filipina singer-songwriter Yeng Constantino, released in the Philippines on January 28, 2013, by Star Music. The album was certified Gold by PARI.[1] The album spawned the singles "B.A.B.A.Y" and hit song "Chinito", and "Sanadata", "Josephine."
Close view of phosphoprotein spots (spots 1-11) differentially expressed (indicated by arrows) during metamorphosis in P. vexillosa. (A): Enlarged phosphoprotein gels (Pre: pre-competent larvae, Com: competent larvae, Juv: juveniles). (B) Relative intensity of the differentially regulated phosphoproteins. (C) Phosphoprotein-to protein intensity ratios.
Close view of phosphoprotein spots (spots 12-21) differentially expressed (indicated by arrows) during metamorphosis in P. vexillosa. (A) Enlarged phosphoprotein gels (Pre: pre-competent larvae, Com: competent larvae, Juv: juveniles). (B) Relative intensity of the differentially regulated phosphoproteins. (C) Phosphoprotein-to-protein intensity ratios.
Close view of phosphoprotein spots (spots 22-32) differentially expressed (indicated by arrows) during metamorphosis in P. vexillosa. (A) Enlarged phosphoprotein gels (Pre: pre-competent larvae, Com: competent larvae, Juv: juveniles). (B) Relative intensity of the differentially regulated phosphoproteins. (C) Phosphoprotein-to-protein intensity ratios.
2-DE Western blot analysis during metamorphosis in P. vexillosa. Two-dimensional gel and immunoblot of tubulin (A) and actin (B) of P.vexillosa (Precom: pre-competent larvae, Com: competent larvae, Juv: juveniles) probed with anti-tubulin and anti-actin monoclonal antibodies and developed by ECL western blotting analysis system.
MHC is also used as a marker of the physiological and developmental states of muscle [28], and it can be involved in the degeneration of the caudal muscle by apoptosis [29], the formation of new muscle (secondary myogenesis) [30], and the conversion of larval muscles to adult muscles [31]. The up-regulation of MHC in COM larvae and its subsequent down-regulation during metamorphosis may suggest the presence of a large proportion of larval myofibers that subsequently disappear during the processes of attaining competency and metamorphosis. Large amounts of myofibrillar proteins need to be synthesized to build up the muscles during metamorphosis.
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PM is the main structural component of the thick filament of smooth muscles. A high abundance of PM in COM larvae of P. vexillosa may indicate that larval metamorphosis is accompanied by a massive reorganization of striated muscles, followed by the development of smooth muscles. The abundant expression of TP, actin-binding protein, could be due to muscle degeneration and differentiation at the onset of larval metamorphosis [32,33].